Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block.
Identifieur interne : 000017 ( Main/Exploration ); précédent : 000016; suivant : 000018Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block.
Auteurs : Vartika Sharma [Inde] ; Muzamil Makhdoomi [Inde] ; Lakshyaveer Singh [Inde] ; Purnima Kumar [Inde] ; Nabab Khan [Inde] ; Sarman Singh [Inde] ; H N Verma [Inde] ; Kalpana Luthra [Inde] ; Sovan Sarkar [Royaume-Uni] ; Dhiraj Kumar [Inde]Source :
- Autophagy [ 1554-8635 ] ; 2020.
Abstract
Opportunistic bacterial infections amongst HIV-infected individuals contribute significantly to HIV-associated mortality. The role of HIV-mediated modulation of innate mechanisms like autophagy in promoting opportunistic infections, however, remains obscure. Here we show, HIV reactivation in or infection of macrophages inhibits autophagy and helps the survival of pathogenic Mycobacterium tuberculosis (Mtb) and nonpathogenic non-tuberculous mycobacterial strains (NTMs). The HIV-mediated impairment of xenophagy flux facilitated bacterial survival. Activation of autophagy by trehalose could induce xenophagy flux and kill intracellular Mtb or NTMs either during single or co-infections. Trehalose, we delineate, activates PIKFYVE leading to TFEB nuclear translocation in MCOLN1-dependent manner to induce autophagy. Remarkably, trehalose significantly reduced HIV-p24 levels in ex-vivo-infected PBMCs or PBMCs from treatment-naive HIV patients and also controlled mycobacterial survival within Mtb-infected animals. To conclude, we report leveraging of HIV-mediated perturbed host innate-immunity by opportunistic bacterial pathogens and show an attractive therapeutic strategy for HIV and associated co-morbidities.Abbreviations: AIDS: acquired immune deficiency syndrome; AMPK: AMP-activated protein kinase; ATG5: autophagy related 5; BafA1: bafilomycin A1; CFU: colony forming unit; CTSD: cathepsin D; CD63: CD63 molecule; EGFP: enhanced green fluorescent protein; FRET: Förster resonance energy transfer; GABARAP: gamma-aminobutyric acid receptor-associated protein; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; GLUT: glucose transporter; HIV: human immunodeficiency virus; hMDMs: human monocyte derived macrophages; IL2: interleukin 2; LAMP1: lysosomal-associated membrane protein 1; LC3B-II: lipidated microtubule-associated proteins 1A/1B light chain 3B; Mtb: Mycobacterium tuberculosis; MTOR: mechanistic target of rapamycin; mRFP: monomeric red fluorescent protein; M6PR: mannose-6-phosphate receptor; NAC: N- acetyl- L -cysteine; NTM's: non-tuberculous mycobacteria; PBMC: Peripheral Blood Mononuclear cells; PIKFYVE: phosphoinositide kinase; FYVE-Type Zinc Finger; PHA: phytohemagglutinin; PMA: phorbol 12-myristate 13-acetate; PtdIns(3,5)P2: Phosphatidylinositol 3,5-bisphosphate; ptfLC3: pEGFP-mRFP-LC3; ROS: reactive oxygen species; SQSTM1: sequestosome1; TFEB: transcription factor EB; MCOLN1/TRPML1: mucolipin 1; PIP4P1/TMEM55B: Human trans-membrane Protein 55B; UVRAG: UV Radiation Resistance Associate; VPS35: vacuolar protein sorting associated protein 35; WDR45: WD repeat domain 45; YCAM: Yellow Chameleon.
DOI: 10.1080/15548627.2020.1725374
PubMed: 32079455
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block.</title><author><name sortKey="Sharma, Vartika" sort="Sharma, Vartika" uniqKey="Sharma V" first="Vartika" last="Sharma">Vartika Sharma</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Makhdoomi, Muzamil" sort="Makhdoomi, Muzamil" uniqKey="Makhdoomi M" first="Muzamil" last="Makhdoomi">Muzamil Makhdoomi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Singh, Lakshyaveer" sort="Singh, Lakshyaveer" uniqKey="Singh L" first="Lakshyaveer" last="Singh">Lakshyaveer Singh</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Purnima" sort="Kumar, Purnima" uniqKey="Kumar P" first="Purnima" last="Kumar">Purnima Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Khan, Nabab" sort="Khan, Nabab" uniqKey="Khan N" first="Nabab" last="Khan">Nabab Khan</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Singh, Sarman" sort="Singh, Sarman" uniqKey="Singh S" first="Sarman" last="Singh">Sarman Singh</name><affiliation wicri:level="1"><nlm:affiliation>Division of Clinical Microbiology & Molecular Medicine, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Clinical Microbiology & Molecular Medicine, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Verma, H N" sort="Verma, H N" uniqKey="Verma H" first="H N" last="Verma">H N Verma</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Jaipur National University, Jaipur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>School of Life Sciences, Jaipur National University, Jaipur</wicri:regionArea><wicri:noRegion>Jaipur</wicri:noRegion></affiliation></author><author><name sortKey="Luthra, Kalpana" sort="Luthra, Kalpana" uniqKey="Luthra K" first="Kalpana" last="Luthra">Kalpana Luthra</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Sarkar, Sovan" sort="Sarkar, Sovan" uniqKey="Sarkar S" first="Sovan" last="Sarkar">Sovan Sarkar</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region></placeName><orgName type="university">Université de Birmingham</orgName></affiliation></author><author><name sortKey="Kumar, Dhiraj" sort="Kumar, Dhiraj" uniqKey="Kumar D" first="Dhiraj" last="Kumar">Dhiraj Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32079455</idno><idno type="pmid">32079455</idno><idno type="doi">10.1080/15548627.2020.1725374</idno><idno type="wicri:Area/Main/Corpus">000115</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000115</idno><idno type="wicri:Area/Main/Curation">000115</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000115</idno><idno type="wicri:Area/Main/Exploration">000115</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block.</title><author><name sortKey="Sharma, Vartika" sort="Sharma, Vartika" uniqKey="Sharma V" first="Vartika" last="Sharma">Vartika Sharma</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Makhdoomi, Muzamil" sort="Makhdoomi, Muzamil" uniqKey="Makhdoomi M" first="Muzamil" last="Makhdoomi">Muzamil Makhdoomi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Singh, Lakshyaveer" sort="Singh, Lakshyaveer" uniqKey="Singh L" first="Lakshyaveer" last="Singh">Lakshyaveer Singh</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Purnima" sort="Kumar, Purnima" uniqKey="Kumar P" first="Purnima" last="Kumar">Purnima Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Khan, Nabab" sort="Khan, Nabab" uniqKey="Khan N" first="Nabab" last="Khan">Nabab Khan</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Singh, Sarman" sort="Singh, Sarman" uniqKey="Singh S" first="Sarman" last="Singh">Sarman Singh</name><affiliation wicri:level="1"><nlm:affiliation>Division of Clinical Microbiology & Molecular Medicine, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Clinical Microbiology & Molecular Medicine, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Verma, H N" sort="Verma, H N" uniqKey="Verma H" first="H N" last="Verma">H N Verma</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Jaipur National University, Jaipur, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>School of Life Sciences, Jaipur National University, Jaipur</wicri:regionArea><wicri:noRegion>Jaipur</wicri:noRegion></affiliation></author><author><name sortKey="Luthra, Kalpana" sort="Luthra, Kalpana" uniqKey="Luthra K" first="Kalpana" last="Luthra">Kalpana Luthra</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Sarkar, Sovan" sort="Sarkar, Sovan" uniqKey="Sarkar S" first="Sovan" last="Sarkar">Sovan Sarkar</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region></placeName><orgName type="university">Université de Birmingham</orgName></affiliation></author><author><name sortKey="Kumar, Dhiraj" sort="Kumar, Dhiraj" uniqKey="Kumar D" first="Dhiraj" last="Kumar">Dhiraj Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Autophagy</title><idno type="eISSN">1554-8635</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Opportunistic bacterial infections amongst HIV-infected individuals contribute significantly to HIV-associated mortality. The role of HIV-mediated modulation of innate mechanisms like autophagy in promoting opportunistic infections, however, remains obscure. Here we show, HIV reactivation in or infection of macrophages inhibits autophagy and helps the survival of pathogenic <i>Mycobacterium tuberculosis (Mtb)</i> and nonpathogenic non-tuberculous mycobacterial strains (NTMs). The HIV-mediated impairment of xenophagy flux facilitated bacterial survival. Activation of autophagy by trehalose could induce xenophagy flux and kill intracellular <i>Mtb</i> or NTMs either during single or co-infections. Trehalose, we delineate, activates PIKFYVE leading to TFEB nuclear translocation in MCOLN1-dependent manner to induce autophagy. Remarkably, trehalose significantly reduced HIV-p24 levels in <i>ex-vivo</i>-infected PBMCs or PBMCs from treatment-naive HIV patients and also controlled mycobacterial survival within <i>Mtb</i>-infected animals. To conclude, we report leveraging of HIV-mediated perturbed host innate-immunity by opportunistic bacterial pathogens and show an attractive therapeutic strategy for HIV and associated co-morbidities.<b>Abbreviations:</b> AIDS: acquired immune deficiency syndrome; AMPK: AMP-activated protein kinase; ATG5: autophagy related 5; BafA1: bafilomycin A<sub>1</sub>; CFU: colony forming unit; CTSD: cathepsin D; CD63: CD63 molecule; EGFP: enhanced green fluorescent protein; FRET: Förster resonance energy transfer; GABARAP: gamma-aminobutyric acid receptor-associated protein; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; GLUT: glucose transporter; HIV: human immunodeficiency virus; hMDMs: human monocyte derived macrophages; IL2: interleukin 2; LAMP1: lysosomal-associated membrane protein 1; LC3B-II: lipidated microtubule-associated proteins 1A/1B light chain 3B; Mtb: <i>Mycobacterium tuberculosis</i>; MTOR: mechanistic target of rapamycin; mRFP: monomeric red fluorescent protein; M6PR: mannose-6-phosphate receptor; NAC: N- acetyl- L -cysteine; NTM's: non-tuberculous mycobacteria; PBMC: Peripheral Blood Mononuclear cells; PIKFYVE: phosphoinositide kinase; FYVE-Type Zinc Finger; PHA: phytohemagglutinin; PMA: phorbol 12-myristate 13-acetate; PtdIns(3,5)P2: Phosphatidylinositol 3,5-bisphosphate; <i>ptfLC3: pEGFP-mRFP-LC3</i>; ROS: reactive oxygen species; SQSTM1: sequestosome1; TFEB: transcription factor EB; MCOLN1/TRPML1: mucolipin 1; PIP4P1/TMEM55B: Human trans-membrane Protein 55B; UVRAG: UV Radiation Resistance Associate; VPS35: vacuolar protein sorting associated protein 35; WDR45: WD repeat domain 45; YCAM: Yellow Chameleon.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32079455</PMID><DateRevised><Year>2020</Year><Month>02</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1554-8635</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Feb</Month><Day>20</Day></PubDate></JournalIssue><Title>Autophagy</Title><ISOAbbreviation>Autophagy</ISOAbbreviation></Journal><ArticleTitle>Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block.</ArticleTitle><Pagination><MedlinePgn>1-20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/15548627.2020.1725374</ELocationID><Abstract><AbstractText>Opportunistic bacterial infections amongst HIV-infected individuals contribute significantly to HIV-associated mortality. The role of HIV-mediated modulation of innate mechanisms like autophagy in promoting opportunistic infections, however, remains obscure. Here we show, HIV reactivation in or infection of macrophages inhibits autophagy and helps the survival of pathogenic <i>Mycobacterium tuberculosis (Mtb)</i> and nonpathogenic non-tuberculous mycobacterial strains (NTMs). The HIV-mediated impairment of xenophagy flux facilitated bacterial survival. Activation of autophagy by trehalose could induce xenophagy flux and kill intracellular <i>Mtb</i> or NTMs either during single or co-infections. Trehalose, we delineate, activates PIKFYVE leading to TFEB nuclear translocation in MCOLN1-dependent manner to induce autophagy. Remarkably, trehalose significantly reduced HIV-p24 levels in <i>ex-vivo</i>-infected PBMCs or PBMCs from treatment-naive HIV patients and also controlled mycobacterial survival within <i>Mtb</i>-infected animals. To conclude, we report leveraging of HIV-mediated perturbed host innate-immunity by opportunistic bacterial pathogens and show an attractive therapeutic strategy for HIV and associated co-morbidities.<b>Abbreviations:</b> AIDS: acquired immune deficiency syndrome; AMPK: AMP-activated protein kinase; ATG5: autophagy related 5; BafA1: bafilomycin A<sub>1</sub>; CFU: colony forming unit; CTSD: cathepsin D; CD63: CD63 molecule; EGFP: enhanced green fluorescent protein; FRET: Förster resonance energy transfer; GABARAP: gamma-aminobutyric acid receptor-associated protein; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; GLUT: glucose transporter; HIV: human immunodeficiency virus; hMDMs: human monocyte derived macrophages; IL2: interleukin 2; LAMP1: lysosomal-associated membrane protein 1; LC3B-II: lipidated microtubule-associated proteins 1A/1B light chain 3B; Mtb: <i>Mycobacterium tuberculosis</i>; MTOR: mechanistic target of rapamycin; mRFP: monomeric red fluorescent protein; M6PR: mannose-6-phosphate receptor; NAC: N- acetyl- L -cysteine; NTM's: non-tuberculous mycobacteria; PBMC: Peripheral Blood Mononuclear cells; PIKFYVE: phosphoinositide kinase; FYVE-Type Zinc Finger; PHA: phytohemagglutinin; PMA: phorbol 12-myristate 13-acetate; PtdIns(3,5)P2: Phosphatidylinositol 3,5-bisphosphate; <i>ptfLC3: pEGFP-mRFP-LC3</i>; ROS: reactive oxygen species; SQSTM1: sequestosome1; TFEB: transcription factor EB; MCOLN1/TRPML1: mucolipin 1; PIP4P1/TMEM55B: Human trans-membrane Protein 55B; UVRAG: UV Radiation Resistance Associate; VPS35: vacuolar protein sorting associated protein 35; WDR45: WD repeat domain 45; YCAM: Yellow Chameleon.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sharma</LastName><ForeName>Vartika</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Makhdoomi</LastName><ForeName>Muzamil</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Singh</LastName><ForeName>Lakshyaveer</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Purnima</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khan</LastName><ForeName>Nabab</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Singh</LastName><ForeName>Sarman</ForeName><Initials>S</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-0749-9647</Identifier><AffiliationInfo><Affiliation>Division of Clinical Microbiology & Molecular Medicine, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Verma</LastName><ForeName>H N</ForeName><Initials>HN</Initials><AffiliationInfo><Affiliation>School of Life Sciences, Jaipur National University, Jaipur, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luthra</LastName><ForeName>Kalpana</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sarkar</LastName><ForeName>Sovan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Dhiraj</ForeName><Initials>D</Initials><Identifier Source="ORCID">https://orcid.org/0000-0001-7578-2930</Identifier><AffiliationInfo><Affiliation>Cellular Immunology Group, International Center for Genetic Engineering and Biotechnology, New Delhi, India.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Autophagy</MedlineTA><NlmUniqueID>101265188</NlmUniqueID><ISSNLinking>1554-8627</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">HIV-TB co-infection</Keyword><Keyword MajorTopicYN="N">mcoln1/TRPML1</Keyword><Keyword MajorTopicYN="N">non-tuberculous mycobacteria</Keyword><Keyword MajorTopicYN="N">opportunistic infection</Keyword><Keyword MajorTopicYN="N">pikfyve</Keyword><Keyword MajorTopicYN="N">trehalose</Keyword><Keyword MajorTopicYN="N">xenophagy</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32079455</ArticleId><ArticleId IdType="doi">10.1080/15548627.2020.1725374</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Midlands de l'Ouest</li></region><settlement><li>Birmingham</li></settlement><orgName><li>Université de Birmingham</li></orgName></list><tree><country name="Inde"><noRegion><name sortKey="Sharma, Vartika" sort="Sharma, Vartika" uniqKey="Sharma V" first="Vartika" last="Sharma">Vartika Sharma</name></noRegion><name sortKey="Khan, Nabab" sort="Khan, Nabab" uniqKey="Khan N" first="Nabab" last="Khan">Nabab Khan</name><name sortKey="Kumar, Dhiraj" sort="Kumar, Dhiraj" uniqKey="Kumar D" first="Dhiraj" last="Kumar">Dhiraj Kumar</name><name sortKey="Kumar, Purnima" sort="Kumar, Purnima" uniqKey="Kumar P" first="Purnima" last="Kumar">Purnima Kumar</name><name sortKey="Luthra, Kalpana" sort="Luthra, Kalpana" uniqKey="Luthra K" first="Kalpana" last="Luthra">Kalpana Luthra</name><name sortKey="Makhdoomi, Muzamil" sort="Makhdoomi, Muzamil" uniqKey="Makhdoomi M" first="Muzamil" last="Makhdoomi">Muzamil Makhdoomi</name><name sortKey="Singh, Lakshyaveer" sort="Singh, Lakshyaveer" uniqKey="Singh L" first="Lakshyaveer" last="Singh">Lakshyaveer Singh</name><name sortKey="Singh, Sarman" sort="Singh, Sarman" uniqKey="Singh S" first="Sarman" last="Singh">Sarman Singh</name><name sortKey="Verma, H N" sort="Verma, H N" uniqKey="Verma H" first="H N" last="Verma">H N Verma</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Sarkar, Sovan" sort="Sarkar, Sovan" uniqKey="Sarkar S" first="Sovan" last="Sarkar">Sovan Sarkar</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000017 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000017 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32079455
|texte= Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32079455" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |